gradient sds polyacrylamide precast gels  (Bio-Rad)

Journal: Nucleic Acids Research

Article Title: Oxidative stress at telomeres triggers internal DNA loops, TRF1 dissociation, and TRF2-dependent R-loops

doi: 10.1093/nar/gkaf285

Figure Lengend Snippet: Accumulation of DNA SSBs at telomeres upon oxidative stress induction in HEK293E cells. Detection of telomeric SSB accumulation at G-rich ( A ) and C-rich ( B ) strands by alkaline gel electrophoresis upon menadione treatment with or without prior treatment with NAC. The signal intensity curves are shown on the right. ( C ) Quantification of DNA damage levels in panels (A) and (B), represented by the percentage decrease in telomeric fragment length of the menadione-treated sample compared to untreated. ( D ) Detection of telomeric SSB accumulation at C-rich strand by alkaline gel electrophoresis upon menadione and H 2 O 2 treatment. The signal intensity curves are shown on the right. ( E ) Quantification of DNA damage levels in panel (D) represented by the percentage decrease in telomeric fragment length of the menadione-treated sample compared untreated. (*) P < .05, ns—not significant. Data represent mean ± SD. n = 3.

Article Snippet: The samples were boiled at 95°C for 10 min, followed by separation on a 4%–15% SDS–polyacrylamide gel electrophoresis (PAGE) precast gel (Mini-PROTEAN TGX Gels, Bio-Rad).

Techniques: Nucleic Acid Electrophoresis